The use of pyrotechnics inside stadiums in Scotland has been illegal since 2023.
Rangers and Celtic have been issued with fines in recent years after incidents at matches in Europe, and Celtic were also warned by Uefa that fans would face a travel ban if they continued to breach the rules.
The football authorities are also working closely with the Scottish government to push for more football banning orders for those charged with pyrotechnic offences.
Football banning orders prevent individuals from attending any match in the UK.
Rangers said in a statement: “Rangers notes the statement from the SPFL and the sanction imposed on the club following the use of pyrotechnics at Premier Sports Cup fixtures earlier this season
“The club has been consistently clear that the use of pyrotechnics at our matches in any competition is unacceptable and could lead to sanctions.
“This has now come to pass with 500 of our supporters to be locked out of a Premier Sports Cup tie at Hampden the next time the club reaches the latter stages of that competition.
“A repeat use at any future SPFL (Premiership or League Cup) game will lead to even more supporters – the vast majority of whom will not have used pyrotechnics – being unable to attend a future semi-final or final tie in the Premier Sports Cup at the national stadium.
“Rangers again reiterates that pyrotechnic use is illegal, and will now deprive Rangers supporters the opportunity to back their team in a key match.”
Celtic and Motherwell have been approached for comment.
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